BLASTN
BLASTN employs the BLAST (Basic Local Alignment Search Tool) heuristic search algorithm. It ascribes significance using the statistical methods of Karlin and Altschul (1990, 1993) with a few enhancements. The BLAST programs were tailored for sequence similarity searching -- for example to identify homologs to a query sequence. The programs are not generally useful for motif- style searching. For a discussion of basic issues in similarity searching of sequence databases, see Altschul et al. (1994). BLASTN compares a nucleic acid query sequence against a nucleic acid sequence database.
Other Blast tools search sequence databases in the following ways:
- blastp compares protein sequences against protein sequence database(s)
- blastx compares the six-frame conceptual translation products of a nucleic acid sequence with a protein sequence database(s)
- tblastn compares a protein sequence with the six-frame conceptual translation products of a nucleic acid sequence database(s)
- tblastx compares the six-frame translations of a nucleic acid sequence with the six-frame translations of a nucleic acid sequence database(s)
Manual: http://www.bru.licr.org/blast/docs/blast_help.html
INPUT = Nucleic Acid Sequences.