PEPSTATS
PEPSTATS reports calculated or predicted physical properties of a protein sequence and its components.
For the entire sequence, the program:
- Calculates molecular weight
- Reports number of residues
- Caculates Average residue weight
- Predicts net charge
- Predicts isoelectric point (pI)
- Predicts whether protein expression in E. coli willoccur in inclusion bodies
- Predicts Molar extinction coefficient at 280 nm (e280) (assumes no cofactor with significant absorbance at 280 nm)
- Predicts absorbance (A>280) at concentration of 1 mg/ml in 1cm pathlength cell; (assumes no cofactor with significant absorbance at 280 nm)
For each type of amino acid, the program calculates:
- number
- mole percent
- DayhoffStat*
For each physico-chemical class of amino acid:
- number
- mole percent
*DayhoffStat is the amino acid's molar percentage divided by the Dayhoff statistic. The Dayhoff statistic is the amino acid's relative occurence per 1000 aa normalised to 100.
If you use EMBOSS, please cite: Rice, P, Longden, I, and Bleasby, A (2000) " EMBOSS: The European Molecular Biology Open Software Suite" Trends in Genetics 16, (6) 276--277. Original program "PEPSTATS"by rls@ebi.ac.uk (original work from 1993).
Manual: http://embossgui.sourceforge.net/demo/manual/pepstats.html
INPUT = Protein Sequences.
TEST INPUT FILES
Input file: pepstats_in.txt
TEST OUTPUT FILES
Output file: pepstats_out.txt